Figure 3
From: eNAMPT neutralization reduces preclinical ARDS severity via rectified NFkB and Akt/mTORC2 signaling
eNAMPT-neutralizing strategies attenuate rat lung injury in preclinical “two-hit” blast trauma/VILI. These studies were conducted at the US Army Institute of Surgical Research (Fort Sam Houston, San Antonio, TX). (A, B) Robust increases in NAMPT staining was observed in lung tissues (as in Fig. 1) from blast/VILI-exposed rats (BOP-150.4 kPa, t + = 3.4 ms; VILI- 4 h, 70 breaths/min, tidal volume 10 ml/kg, 0 PEEP) with quantification by Image J software. *p < 0.05. (C, D) Microscopic H&E lung tissue staining in blast/VILI-challenged rats demonstrated profound inflammatory lung injury with increased PMN infiltration, alveolar septal thickening, edema, and hemorrhage (*p < 0.05 pAb Blast/VILI vs. untreated Blast/VILI) which was significantly reduced in rats receiving the eNAMPT-neutralizing pAb (4 mg/kg) delivered at time 0.5 h post blast exposure, but prior to mechanical ventilation.
