Table 1 Primer sequences.
Accession number | Primers | Sequences | Size (nt) | Tm (℃) | Annealing temperature (℃) |
|---|---|---|---|---|---|
NM_030994 | Itga1-qPCR-F | ACCAGTCAGCAGCTTCATTT | 20 | 55 | 60 |
Itga1-qPCR-R | CACAGTTCCGTTCCAGTCATAG | 22 | 57 | ||
NM_053889 | Vwf-qPCR-F | TGGTCACACTCTCACATTTACTC | 23 | 54 | 60 |
Vwf-qPCR-R | CACAGATTCCACAGAGACCATAC | 23 | 56 | ||
NM_001169138 | Thbs-qPCR-F | ACAGGCATAGGCTCGATTTATT | 22 | 58 | 60 |
Thbs-qPCR-R | CTGAACTCATGTGGAGTGAGAG | 22 | 54 | ||
NM_001108237 | Laminin-qPCR-F | CATCGTTCCACGGGTGTATTA | 21 | 58 | 60 |
Laminin-qPCR-F | GCATGTGTCCAGCTCTACTT | 20 | 52 | ||
NM_053304 | Collagen-qPCR-F-rat | GCTTGAAGACCTATGTGGGTATAA | 24 | 58 | 60 |
Collagen-qPCR-R-rat | GGGTGGAGAAAGGAACAGAAA | 21 | 58 | ||
NM_017008 | Gapdh-qPCR-F | GCAAGGATACTGAGAGCAAGAG | 22 | 56 | 60 |
Gapdh-qPCR-R | GGATGGAATTGTGAGGGAGATG | 22 | 61 |
