Figure 4

NKA-α, SLC26A6 positivity rate in the mid-intestine of Gymnocypris przewalskii at different time points exposed to saline-alkaline lake water with a salinity of 17 (L17). Light–dark cycle was 14:10-h light:dark (5:00–19:00 with light intensity of 600–1000 lx;19:00–5:00 with light intensity of 0 lx); Lowercase indicates significant difference among time points in each treatment (P < 0.05); Positive rate = protein staining/nuclear staining. Image J was used for measurement, analysis and calculation).