Figure 5 | Scientific Reports

Figure 5

From: A high-throughput cell-based assay pipeline for the preclinical development of bacterial DsbA inhibitors as antivirulence therapeutics

Figure 5

Absorbance-based monitoring of bacterial motility. (A) Motility curves of E. coli JCB816 and E. coli ΔdsbA JCB817 monitored spectrophotometrically during incubation on soft LB agar at 37 °C over 13 h. (B) Digital images tracking the swimming motility of E. coli JCB816 on soft LB agar in a 24-well plate. E. coli was inoculated at the left edge of each well, and by 12 h incubation at 37 °C the zone of motility (boundary marked in red) had reached the opposite edge of the well. (C) Motility curves of P. aeruginosa (PAO1) and S. Typhimurium (SL1344) monitored spectrophotometrically during incubation on soft LB agar at 37 °C over 15 h. Data are shown as absorbance at 600 nm (A and C), with mean ± SD of 3 biological replicates plotted at each time point.

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