Table 1 Motility curve parameters for UPEC CFT073 in the presence of varying concentrations of DsbA inhibitor F1.

From: A high-throughput cell-based assay pipeline for the preclinical development of bacterial DsbA inhibitors as antivirulence therapeutics

F1

(mM)

Start of motility

(h)

End of motility

(h)

Rate of motility

(slope)a

Inhibition of motility (%)b

1.00

11

n/d

0.02 ± 0.007

97

0.75

7

n/d

0.09 ± 0.013

89

0.50

6

12

0.21 ± 0.02

51

0.25

5

11

0.28 ± 0.02

30

0.10

5

11

0.33 ± 0.01

17

0.00

5

11

0.38 ± 0.03

n/a

  1. aMotility rates shown as mean slope value ± S.D. from four biological replicates. Group means were compared using the one-way ANOVA test (P < 0.0001).
  2. bCompared to vehicle control (DMSO 0.4%) and determined using data from the 10-h time point.
  3. n/d = not determined.
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