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Figure 3

From: Pannexin 3 regulates skin development via Epiprofin

Figure 3

Panx3 regulates HaCaT cell differentiation. (A) Immunostaining of Panx3 (green), Filaggrin (red), and Notch1 (purple), Ki67 (yellow) in pCMV6-mock or pCMV6-Panx3 transfected HaCaT cells cultured in normal condition media (a). Relative fluorescence intensity comparison of images and % of Ki67 positive cells were analyzed (b). *p < 0.01. Error bars represent the mean ± SD; N = 3. The nuclei were counterstained with DAPI (blue in merged). (B) qPCR for K1, K10, K5, and K14 in pCMV6-mock or pCMV6-Panx3 transfected HaCaT cells. Results represent the mean ± SD; N = 3. *p < 0.01.

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