Figure 7
Panx3 ER Ca2+ channel regulates Epfn via Akt/NFAT signaling pathway. HaCaT cells were cultured for 3 days in normal media. Western blotting was performed with antibodies to Panx3, Epfn, Notch1, phospho-Akt (P-Akt), Akt, phospho-NFATc1 (P-NFATc1), NFATc1, and α-Tubulin. The cropped blots were used in the figures. Full blot is shown in the Supplementary Information (Full Original Blots-I–IV). (B) Quantification of the ratios of Epfn/α-Tubulin (upper panel of A,b and B,b) and Notch1/α-Tubulin (lower panel of A,b and B,b), P-Akt/Akt (upper panel of A,c and B,c) and P-NFATc1/NFATc1 (lower panel of A,c and b,c). **p < 0.05, *p < 0.01. Error bars represent the mean ± SD; N = 3.
