Figure 5

Hsa-miR-494-3p binds to 3ā²-UTR of HtrA3. (A) A target binding site between the 3ā²-UTR of HtrA3 and hsa-miR-494-3p; (B) Luciferase activity was significantly decreased in HK2 cells co-transfected hsa-miR-494-3p with wild-type 3ā²-UTR of HtrA3, but not in cells transfected with mutant 3ā²-UTR of HtrA3. N (the replicate for above experiment)ā=ā3. *Pā<ā0.05, **Pā<ā0.01, ***Pā<ā0.001, versus control. #Pā<ā0.05, ##Pā<ā0.01, ###Pā<ā0.001, versus hypoxia/reoxygenation treatment group.