Figure 7

Overexpression of HtrA3 reduced ischemia/reperfusion-induced inflammatory response in HK2 cells. (A) Real-time PCR data show overexpression of HtrA3 decreased ischemia/reperfusion-induced mRNA of TNF-α and IL-6; (B) ELISA data show overexpression of HtrA3 decreased ischemia/reperfusion-induced TNF-α and IL-6 protein level; (C) Western blot data show overexpression of HtrA3 decreased ischemia/reperfusion-induced NF-κB phosphorylation. N (the replicate for above experiments) = 3. *P < 0.05, **P < 0.01, ***P < 0.001, versus control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, versus hypoxia/reoxygenation treatment group.