Figure 2 | Scientific Reports

Figure 2

From: Integrated, automated maintenance, expansion and differentiation of 2D and 3D patient-derived cellular models for high throughput drug screening

Figure 2

Quality control of cell seeding. (a) Fibroblast images taken with the multimode plate reader to determine confluency without (top) and with (bottom) mask highlighting areas covered by cells. (b) Acquisition mask for confluency measurement of a 1-well plate (top) and a 6-well plate (bottom). Each field represents a field of acquisition and numbers in the fields indicate percentage of confluency. (c–e) Non-linear curve fitting on confluency reads of serially diluted or split fibroblasts (n = 3) (c), smNPC (n = 7) (d), and iPSC (n = 4) (e). (f) Measurement of CV after dispensing test with fibroblast into dry or pre-wetted 6-wells. n = 3. (g) Measurement of CV after testing dispensing speeds with fibroblast in 6-wells. n = 6 (h) Measurement of CV after testing dispensing speeds with iPSC in 6-wells. n = 9, statistical test used: Mann–Whitney test.

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