Figure 4
PIXI analysis preserves the correlations between the number of plated cells and XF assay parameters. (a–j) Comparison of the linear relationship between initial OCR, basal OCR, FCCP-uncoupled OCR, basal GR or monensin-induced GR and number of plated cells (a–e) or number of PIXI analyzed cells (f–j) (50–300 × 103 cells/well, n = 14–16). Means at each seeding density (a–d) are represented by a dash (⎯). (k–o) Comparison of mean OCR and GR levels between donor A, B and C at 200,000 plated cells (left) or at 200,000 PIXI analyzed cells (right) per well (n = 16). Data is represented as mean ± range. Means are represented by a plus sign (+). ns not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
