Table 2 Effects of PIXI analysis on the variation between PBMC donors.

From: Novel standardized method for extracellular flux analysis of oxidative and glycolytic metabolism in peripheral blood mononuclear cells

 

Basal OCR

Basal GR

mean ± s.d. (pmol/min)

CV (%)

mean ± s.d. (mpH/min)

CV (%)

Standard analysis

28.96 ± 5.11

17.7

6.38 ± 1.20

18.8

PIXI analysis

26.69 ± 3.74

14.0

5.94 ± 1.11

18.7

Effect of PIXI analysis

− 2.27 ± − 1.37

− 3.7

− 0.44 ± − 0.09

− 0.1

  1. PBMCs were isolated from sixteen healthy lean individuals (females, 18–25 years of age, BMI 18.5–25 kg/m2) and plated at 75–300 × 103 cells/well (n = 3–4) to generate a calibration curve that was used to transform total pixel intensity values into PIXI analyzed cell numbers. XF assay measurements were performed at 225 × 103 cells/well (n = 8) and basal OCR and basal GR levels were normalized against the number of plated cells (standard analysis) or PIXI analyzed cells (PIXI analysis). Here a summary of the changes in group mean, s.d. and CV for basal OCR and basal GR after standard analysis or PIXI analysis is represented.
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