Table 2 DNA damage in cells from the liver, brain, bone marrow and testes (sperms) extracted from mice chronic administered with LPS/D-GalN.

From: Co-administration of lipopolysaccharide and d-galactosamine induces genotoxicity in mouse liver

 

Tail DNA (%)

Tail moment

Olive tail moment

Liver cell

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 0.9% NaCl

1.4161 ± 0.90

0.46 ± 0.11

0.25 ± 0.10

LPS/D-GalN

 10 µg/kg + 100 mg/kg

31.8718 ± 9.86***

33.50 ± 3.64***

21.22 ± 8.33***

CTX

 50 mg/kg

23.4090 ± 8.89***

20.16 ± 1.87***

10.45 ± 4.34***

Brain cell

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 0.9% NaCl

2.96 ± 0.38

0.42 ± 0.10

0.69 ± 0.10

LPS/D-GalN

 10 µg/kg + 100 mg/kg

38.42 ± 16.52***

36.51 ± 4.93***

20.72 ± 2.48***

CTX

 50 mg/kg

26.76 ± 11.15***

19.90 ± 1.87***

11.12 ± 0.91***

Bone marrow cell

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 0.9% NaCl

0.94 ± 0.16

0.04 ± 0.01

0.20 ± 0.02

LPS/D-GalN

 10 µg/kg + 100 mg/kg

1.88 ± 0.08

0.18 ± 0.03

0.44 ± 0.06

CTX

 50 mg/kg

9.08 ± 0.13***

1.58 ± 0.26***

2.62 ± 0.33***

Sperm cell

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 0.9% NaCl

3.75 ± 0.59

0.47 ± 0.08

0.94 ± 0.16

LPS/D-GalN

 10 µg/kg + 100 mg/kg

2.68 ± 0.41

0.25 ± 0.05

0.58 ± 0.09

CTX

 50 mg/kg

7.46 ± 0.51**

1.58 ± 0.26**

1.90 ± 0.17**

  1. Mice were peritoneally injected with LPS/D-GalN (10 μg/kg + 100 mg/kg body weight) every other day for 8 weeks. Saline solution (0.9%) and cyclophosphamide (CTX) (50 mg/kg body weight) given in the same mode as described above were used as negative and positive control, respectively. SCGE assay was performed using cells from each tissue. Data are expressed as means ± S.D.; *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the control group.
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