Figure 4

Colonic ENaC expression and fecal Na⁺ excretion are also altered in N5/6 HKA2KO mice. (A) Daily urine aldosterone excretion in N5/6 WT (white squares) and N5/6 HKA2KO (black squares) mice under normal (NK) or high K⁺ intake (2% KCl). Results are shown as the mean ± s.e.m, n = 5. (B–D) mRNA expression of ENaC α, β and γ subunits in N5/6 WT (white squares, n = 10) and N5/6 HKA2KO (black squares, n = 10) mice under normal (NK) or high K⁺ intake (2% KCl). Results are shown as the mean ± s.e.m. (E) higher panel, Immunoblot of the ENaC β subunit in N5/6 WT (white squares, n = 4) and N5/6 HKA2KO (black squares, n = 4) mice under normal (NK) or high K⁺ intake (2% KCl). Middle panel, Ponceau red labeling of the membrane. Lower panel, quantification of the band intensity normalized with the Ponceau red labeling. (F) Fecal excretion of Na⁺ in N5/6 mice under normal (NK) or high K⁺ intake (2% KCl). (G) Urine excretion of Na⁺ in N5/6 mice under normal (NK) or high K⁺ intake (2% KCl). Results are shown as the mean ± s.e.m. Numbers in italic = n of mice. Two-way ANOVA test followed by a Sidak’s multiple comparisons test, (**p < 0.01; *p < 0.05).