Figure 1
From: Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression
Identification of Lin28a interactors during EpiLC formation. (a) Chromatogram (left) showing the elution profile of the size-exclusion chromatography. ESCs were transfected with Lin28a-Flag expressing construct at 1d of EpiLCs transition and the protein extracts obtained at 3d EpiLCs were loaded on a Superose 6 Increase 10/300 GL column. Western blot (right) analysis of column fractions with the anti-Flag antibody shows the presence of ectopic Lin28a protein in specific fractions. (b) GO terms (biological process and molecular function) identified using DAVID software for the 201 proteins specifically found in Lin28 immunoprecipitates by proteomic analysis. (c) Venn diagram comparing Lin28 co-purified proteins with lists of proteins interacting directly or indirectly with RNA molecules. HeLa RNPs, HEK293 RNPs and ESC RNPs: proteins found in ribonucleoprotein particle of HeLa, HEK293 and ESC cell lines respectively; miRNA BPs miRNA binding proteins, P-bodies protein found in P-bodies of HEK293 and HeLa cells, stress granule proteins found in mammalian cells.
