Table 4 Recovery values (as SP-enol) and coefficients of variation obtained with fortified samples by the developed direct cELISA (n = 3).
Sample | [A] | SP | SP-enol | SP + SP-enol (1:1) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
d.f | R (%) | CV (%) | d.f | R (%) | CV (%) | d.f | R (%) | CV (%) | ||
Grapes | 2.5 | 25 | – | – | 25 | – | – | 25 | – | – |
5.0 | 25 | 113.9 | 10.3 | 25 | 121.7 | 8.3 | 25 | 121.3 | 7.3 | |
10 | 25 | 99.7 | 9.3 | 25 | 113.4 | 12.0 | 25 | 113.9 | 18.3 | |
25 | 25 | 107.2 | 12.8 | 25 | 112.9 | 5.5 | 25 | 116.5 | 14.2 | |
50 | 50 | 92.7 | 6.6 | 50 | 99.9 | 7.1 | 50 | 94.2 | 17.1 | |
100 | 50 | 98.1 | 10.9 | 50 | 99.6 | 8.3 | 50 | 98.8 | 18.9 | |
250 | 50 | 85.6 | 8.5 | 50 | 98.3 | 7.1 | 50 | 91.2 | 13.9 | |
500 | 50 | 83.7 | 1.7 | 50 | 96.2 | 9.6 | 50 | 91.9 | 14.6 | |
White grape juice | 2.5 | 5 | 88.4 | 2.7 | 5 | 116.5 | 4.8 | 5 | 81.5 | 6.3 |
5.0 | 5 | 90.6 | 4.5 | 5 | 113.6 | 6.8 | 5 | 84.9 | 2.2 | |
10 | 5 | 98.5 | 13.4 | 5 | 114.0 | 5.8 | 5 | 88.7 | 10.8 | |
25 | 5 | 99.9 | 11.5 | 5 | 112.5 | 10.3 | 5 | 88.5 | 17.3 | |
50 | 50 | 102.0 | 6.5 | 50 | 106.4 | 5.2 | 50 | 83.7 | 3.1 | |
100 | 50 | 115.3 | 11.3 | 50 | 109.3 | 8.6 | 50 | 89.4 | 9.1 | |
250 | 50 | 102.8 | 10.8 | 50 | 97.0 | 7.5 | 50 | 83.8 | 13.9 | |
500 | 50 | 85.4 | 8.4 | 50 | 97.3 | 10.8 | 50 | 89.8 | 6.8 | |
Red grape juice | 2.5 | 5 | 98.4 | 5.2 | 5 | 112.9 | 1.7 | 5 | 92.3 | 15.3 |
5.0 | 5 | 92.0 | 4.3 | 5 | 115.2 | 2.7 | 5 | 88.5 | 15.6 | |
10 | 5 | 101.5 | 8.0 | 5 | 115.5 | 3.7 | 5 | 87.8 | 6.5 | |
25 | 5 | 103.0 | 4.7 | 5 | 118.0 | 1.3 | 5 | 82.8 | 7.1 | |
50 | 50 | 109.9 | 9.8 | 50 | 106.6 | 2.2 | 50 | 88.5 | 7.2 | |
100 | 50 | 109.6 | 3.4 | 50 | 105.5 | 3.5 | 50 | 89.7 | 2.8 | |
250 | 50 | 103.7 | 3.0 | 50 | 103.3 | 1.4 | 50 | 82.8 | 8.7 | |
500 | 50 | 86.1 | 3.0 | 50 | 115.0 | 3.7 | 50 | 85.4 | 2.0 | |
White wine | 2.5 | 5 | 115.4 | 2.9 | 5 | 113.2 | 10.3 | 5 | 115.9 | 5.6 |
5.0 | 5 | 106.8 | 5.9 | 5 | 113.9 | 11.3 | 5 | 104.3 | 16.6 | |
10 | 5 | 106.6 | 2.2 | 5 | 115.3 | 14.1 | 5 | 89.6 | 13.4 | |
25 | 5 | 100.8 | 9.2 | 5 | 105.4 | 8.5 | 5 | 82.9 | 4.5 | |
50 | 50 | 93.2 | 8.8 | 50 | 104.5 | 10.9 | 50 | 95.0 | 2.5 | |
100 | 50 | 102.1 | 4.3 | 50 | 103.4 | 6.1 | 50 | 88.3 | 7.5 | |
250 | 50 | 93.3 | 3.8 | 50 | 104.7 | 2.0 | 50 | 81.1 | 6.4 | |
500 | 50 | 86.2 | 10.3 | 50 | 91.1 | 1.2 | 50 | 81.7 | 9.0 | |
Red wine | 2.5 | 5 | 108.3 | 10.1 | 5 | 107.8 | 14.7 | 5 | 123.4 | 5.9 |
5.0 | 5 | 97.6 | 11.4 | 5 | 107.2 | 15.4 | 5 | 117.9 | 16.8 | |
10 | 5 | 96.9 | 14.2 | 5 | 113.3 | 18.6 | 5 | 103.1 | 8.7 | |
25 | 5 | 93.0 | 10.8 | 5 | 109.7 | 5.6 | 5 | 92.6 | 8.7 | |
50 | 50 | 89.6 | 14.2 | 50 | 96.9 | 14.2 | 50 | 101.0 | 10.9 | |
100 | 50 | 91.7 | 10.5 | 50 | 104.3 | 10.5 | 50 | 98.5 | 9.9 | |
250 | 50 | 88.2 | 11.3 | 50 | 107.3 | 18.1 | 50 | 94.0 | 10.8 | |
500 | 50 | 88.1 | 2.3 | 50 | 98.4 | 1.3 | 50 | 94.5 | 20.0 | |
