Figure 1
From: Elevated inflammatory gene expression in intervertebral disc tissues in mice with ADAM8 inactivated
ADAM8 cleaves fibronectin, and Adam8EQ mutation abrogates its enzymatic activity. MW molecular weight markers; Lane 1: human plasma FN-f (control); Lane 2: hAF, human annulus fibrosus (AF) tissue lysate (control); Lanes 3&4: mouse nucleus pulposus (mNP) & mAF from Adam8EQ mice; Lanes 5&6: mNP and mAF tissues from wild type (WT) mice. (A) membrane probed with an antibody recognizing the FN neoepitope (VRAA; shown in red); (B) probed with monoclonal antibody (MAB) 1936 recognizing the FN N-terminal domain (shown in green); (C) panels (A,B) superimposed. (D) schematic drawing of the FN-f resulting from trypsin or ADAM8 digestion.
