Figure 7

Enlargement of immunohistochemical fluorescence microscopy of the distributions and abundance of carbonic anhydrase, calmodulin and mucus within tagged polyp tissues of O. faveolata. (A) Original enlarged image (from box 7A-B shown in Fig. 6A) of a horizontal cross-section of decalcified polyp tissues of O. faveolata collected in March 2008 exhibiting superimposed emissions from mucus (blue fluorescence), carbonic anhydrase (green fluorescence), and calmodulin (red fluorescence). (B) Line tracing of A indicating individual Symbiodiniaceae. (C) A second enlargement (from area shown in Supplementary Figs. S5, S6) image of a horizontal cross-section of decalcified polyp tissues of O. faveolata collected in May 2008 exhibiting superimposed emissions from mucus (blue fluorescence), carbonic anhydrase (green fluorescence), and calmodulin (red fluorescence). (D) Line tracing of C indicating individual Symbiodiniaceae are found in both the oral endoderm and the calicoblastic epithelium. Labels include: End—endoderm; Mes—mesoglea; Ect—ectoderm; Muc—mucus; PS—primary septum; SW—seawater at the time the polyps were collected; and Sk—skeleton. Tissue structure terminology follows nomenclature in Allemand et al⁵⁴ and Peters⁵⁵.