Figure 6 | Scientific Reports

Figure 6

From: The combination of the tubulin binding small molecule PTC596 and proteasome inhibitors suppresses the growth of myeloma cells

Figure 6

Endoplasmic reticulum stress is augmented by the combination of PTC596 and bortezomib. (A) Venn diagram of 9110 genes upregulated upon PTC596 treatment and 8662 genes upregulated upon bortezomib treatment, compared with the control, in RNA-seq analysis. Gene ontology terms related to endoplasmic reticulum were significantly enriched in 5299 overlapping genes. (B) Quantitative RT-PCR analysis of BMI1 in MM.1S treated with or without PTC596 (100 nM) for 48 h in the presence or absence of bortezomib (2.5 nM) for 24 h. ACTB was used to normalize the amount of input RNA. Data are shown as mean ± SD (n = 3). **P < 0.01; ***P < 0.001; ns, not significant using one-way ANOVA. (C) Western blotting analysis of the indicated proteins in MM.1S. MM.1S were treated with or without PTC596 (100 nM) for 48 h in the presence or absence of bortezomib (2.5 nM) for 24 h. GAPDH served as a loading control. The right graphs show the density volume normalized by GAPDH.

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