Figure 5
Screen of possible downstream transcriptional factors targeted by intestinal linc-50 in simulated microgravity treated nematodes. (a) DAF-16, SKN-1, and HLH-30 are candidate transcriptional factors targeted by linc-50 in simulated microgravity treated nematodes. TFs, transcriptional factors. (b) Effect of simulated microgravity on expressions of daf-16, skn-1, and hlh-30. N = 3. Bars represent means ± SD. **P < 0.01 vs control. (c) Effect of intestine-specific RNAi knockdown of linc-50 on expressions of daf-16, skn-1, and hlh-30 in simulated microgravity treated nematodes. N = 3. L4440, empty vector. Bars represent means ± SD. **P < 0.01 vs VP303. Simulated microgravity treatment was performed in RCCS system at 30 rpm for 24 h.
