Figure 5
Sensitive and selective detection of GSH using FA-rGO-BSA/AuNCs. (a) Fluorescence intensity of FA-rGO- BSA/AuNCs by varying the concentrations of GSH. The relative concentrations of GSH were 0, 2, 4, 6, 8, 10, 12, 14 and 16 µg mL−1 at λex = 365 nm. (b) Relationship between the fluorescence quenching values (F0 − F)/F0 and the target concentrations. (c) Linear response of the fluorescence quenching values (F0 − F)/F0 to the concentration of GSH. Error bars are the standard deviation of three repetitive experiments. (d) Selective detection of 0.5 mM GSH in the presence of potentially interfering components. The concentrations of glycine, proline, leucine, methionine, fructose, glucose, tryptophan, NaCl, KCl, CaCl2, MgSO4 and MnCl2·4H2O were 5 mM. The concentration of ascorbic acid was 50 mM. F0 and F are the fluorescence intensities of the sensing system in the absence and presence of GSH (or other potentially interfering chemicals), respectively. Error bars indicate the standard deviation of three independent measurements.
