Table 5 Genetic diversity analysis of three dragon fruit (Hylocereus) species grown under Andaman and Nicobar Island conditions using ISSR markers.
Sl. no. | Primers | Sequence (5′–3′) | T °C | Total number of bands amplified | No. of monomorphic bands | No. of polymorphic bands | % Polymorphism | PIC |
|---|---|---|---|---|---|---|---|---|
1 | UBC807 | AGAGAGAGAGAGAGAGT | 57.0 | 15 | 2 | 13 | 86.7 | 0.68 |
2 | UBC810 | GAGAGAGAGAGAGAGAT | 48.0 | 14 | 1 | 13 | 92.8 | 0.66 |
3 | UBC811 | GAGAGAGAGAGAGAGAC | 55.0 | 19 | 6 | 13 | 68.4 | 0.45 |
4 | UBC824 | TCTCTCTCTCTCTCTCG | 50.0 | 14 | 3 | 11 | 78.6 | 0.58 |
5 | UBC835 | AGAGAGAGAGAGAGAGYC | 60.0 | 14 | 4 | 10 | 71.4 | 0.44 |
6 | UBC840 | GAGAGAGAGAGAGAGAYT | 53.0 | 15 | 4 | 11 | 73.3 | 0.45 |
7 | UBC848 | CAC ACACACACACACARG | 58.0 | 12 | 2 | 10 | 83.3 | 0.54 |
8 | UBC880 | GGAGAGGAGAGG AGA | 55.0 | 9 | 2 | 7 | 77.8 | 0.63 |
9 | UBC887 | DVDTCTCTCTCTCTCTC | 48.0 | 5 | 4 | 1 | 20.0 | 0.51 |
10 | UBC895 | AGAGTTGGTAGCTCTTGATC | 48.0 | 17 | 6 | 11 | 64.7 | 0.42 |
11 | UBC900 | ACTTCCCCACAGGTTAACACA | 50.0 | 14 | 3 | 11 | 78.6 | 0.48 |
12 | UBC815* | CTCTCTCTCTCTCTCTG | 56.0 | 9 | 0 | 6 | 66.6 | 0.88 |
13 | UBC856* | ACACACACACACACA CYA | 55.0 | 13 | 0 | 11 | 84.6 | 0.91 |
14 | UBC891* | HVHTGTGTGTGTGTGTG | 50.0 | 8 | 0 | 4 | 50.0 | 0.84 |
15 | UBC825+ | ACACACACACACACACT | 54.0 | – | – | – | – | – |
16 | UBC853+ | TCTCTCTCTCTC TCTCRT | 52.7 | – | – | – | – | – |
Sum | 178 | 37 | 132 | – | – | |||
Average | 12.7 | 2.64 | 9.43 | 71.2 | – | |||
Range | 5–19 | 0–6 | 1–13 | 20.0–92.8 | 0.42–0.91 | |||
