Figure 1
From: Direct current stimulation enhances neuronal alpha-synuclein degradation in vitro
Effects of DCS on protein (A,B) and gene (C) expression of asyn, TDP-43 and BDNF (mRNA levels only) in SH-SY5Y cells under basal conditions at the end of stimulation (R0), after 1 and 17 h (R1 and R17). Reduced oligomeric/aggregated and increased monomeric asyn forms were found in DCS-treated cells (A,B). (B) Representative Western blot image showing immunoreactivity at different time points (R0, R1 and R17) for monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms, TDP-43 and GAPDH, used as internal standard, in cells exposed to DCS or sham. Full-length blots are presented in Supplementary M&M. (N = 3; **p < 0.01, *p < 0.05 vs. sham).
