Figure 2

Effects of DCS on protein and gene expression of macroautophagy markers (Beclin-1, LC3, p62) (A,C) and on the activation/phosphorylation of 2 regulatory pathways (Erk1/2, Akt) (D,E) in SH-SY5Y cells under basal conditions at the end of stimulation (R0), after 1 and 17 h (R1 and R17). A down-regulation of macroautophagy was found in DCS-treated cells. (B) Representative Western blot image showing immunoreactivity at different time points (R0, R1 and R17) for p62, Beclin-1, LC3 and β-actin, used as internal standard, in cells exposed to DCS or sham. Full-length blots are presented in Supplementary M&M. (E) Representative Western blot image showing immunoreactivity at different time points (R0, R1 and R17) for phosho-Akt, phospho-Erk1/2 and GAPDH, used as internal standard, in cells exposed to DCS or sham. Full-length blot for phospho-Akt and available cropped blot for phospho-Erk1/2 are presented in Supplementary M&M. (N = 3; **p < 0.01, *p < 0.05 vs. sham).