Figure 4

Effect of rotenone (400 nM, 24 h) or NH₄Cl (10 mM, 24 h) on protein expression of monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms (A) and asyn gene expression (C). Increased monomeric asyn was found in rotenone- or NH₄Cl-treated cells (A,B) and increased asyn gene transcription was found in rotenone-treated cells (C). (B) Representative Western blot image showing immunoreactivity for monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms and β-actin, used as internal standard, in vehicle-treated cells (VEH) and cells exposed to rotenone (400 nM, 24 h) or NH₄Cl (10 mM, 24 h). Full-length blot is presented in Supplementary M&M. (N = 3; *p < 0.05 vs. vehicle).