Figure 5

Effects of DCS on protein expression of monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms in cells treated with rotenone (400 nM, 24 h); cells were collected 17 h after DCS (R17); the effect of rotenone alone on asyn was also shown. DCS counteracted the rotenone-induced increase of monomeric asyn and reduced oligomeric/aggregated asyn forms (A,B). (B) Representative Western blot image showing immunoreactivity for monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms and β-actin, used as internal standard, in cells exposed to sham and cells treated with rotenone alone or rotenone and DCS. Full-length blot is presented in Supplementary M&M. (N = 3; **p < 0.01, *p < 0.05 vs. untreated cells, °p < 0.05 vs. rotenone-treated cells).