Figure 6
From: Direct current stimulation enhances neuronal alpha-synuclein degradation in vitro
Effects of DCS on protein expression of macroautophagy (A–C) and CMA (B,C) markers in cells treated with rotenone (400 nM, 24 h); cells were collected 17 h after DCS (R17). An up-regulation of macroautophagy (A–C) and CMA (B,C) was found in rotenone-treated cells after DCS. (C) Representative Western blot images showing immunoreactivity for Beclin-1, LC3-I/II, LAMP-2A, HSC70, p62 and MEF2D and β-actin, used as internal standard, in sham-treated cells and cells exposed to rotenone alone or rotenone and DCS. Full-length blot or details on the used methodological approach are included in Supplementary M&M. (N = 3; *p < 0.05 vs. untreated cells, °p < 0.05 vs. rotenone-treated cells).
