Figure 7

Effects of DCS on protein expression of monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms in cells treated with NH₄Cl (10 mM, 24 h); cells were collected 17 h after DCS (R17); the effect of NH₄Cl alone on asyn was also shown. DCS counteracted the NH₄Cl-induced increase of monomeric asyn (A,B). (B) Representative Western blot image showing immunoreactivity for monomeric (19 kDa) and oligomeric/aggregated (50 and 100 kDa) asyn forms and β-actin, used as internal standard, in cells exposed to sham and cells treated with NH₄Cl alone or NH₄Cl and DCS. Blot details and available images are included in Supplementary M&M. (N = 3; *p < 0.05 vs. untreated cells, °p < 0.05 vs. NH₄Cl-treated cells).