Figure 8

Effect of DCS on extracellular soluble asyn levels in culture media of cells treated with NH₄Cl (10 mM, 24 h); culture media were collected 17 h after DCS (R17); the effect of NH₄Cl alone on extracellular soluble asyn was also shown. DCS counteracted the NH₄Cl-induced increase of extracellular levels of soluble asyn (A,B). (A) Histogram showing the levels of extracellular soluble asyn expressed as optical density (OD) in cells treated with NH₄Cl alone or NH₄Cl and DCS. (B) Representative Dot blot image showing immunoreactivity for extracellular soluble asyn in cells exposed to sham and cells treated with NH₄Cl alone or NH₄Cl and DCS (volume range: 50–12.5 ul). Full-length blot is presented in Supplementary M&M. (N = 3; *p < 0.05 vs. sham).