Figure 3
HSA is an essential component for the differential expression of genes in A. baumannii. A. baumannii A118 cells were cultured in LB or LB supplemented with one of the following: 4% CSF, HSA-depleted CSF (dCSF), or dCSF + 0.2% HSA and its cDNA was synthesized. RT-qPCR was conducted with three genes (A) fimA encoding gene, (B) vgrG1, and (C) bauD. Shown are the means of the results obtained from three independent experiments. The y axis refers to the fold difference of each gene to the threshold cycle (CT) values corresponding to recA; the standard deviation SD is shown. Asterisks indicate significant differences among treatments, as determined by ANOVA followed by Tukey’s multiple comparison test (P < 0.05).
