Figure 3

Limit of detection of chimeric A/B standards. (a) Amplification curves of targets N1, N2, N3 and RP in 100-fold serial dilutions of standards A and B against the human universal RNA, with three technical replicates. (b) Observed Ct values for targets N1, N2, N3 and RP at different dilutions of standards A and B (100, 10–2, 10–4 and 10–6), across three technical replicates.