Figure 2 | Scientific Reports

Figure 2

From: Anti-CfaE nanobodies provide broad cross-protection against major pathogenic enterotoxigenic Escherichia coli strains, with implications for vaccine design

Figure 2

Selected anti-CfaE nanobodies show cross-reactivity against class 5 colonization factors. (A–D) Two yeast-derived clones (2R215 and 2R23) and two llama-derived clones (1D7 and 1H4) were examined by ELISA for binding to eight class 5 adhesins. These clones showed broad reactivity against multiple adhesins. Three biological replicates were performed. (E) The four lead nanobodies were tested for inhibition of mannose resistant hemagglutination (MRHA) against an ETEC strain expressing CFA/I (H10407). All nanobodies showed maximal inhibitory concentration (IC100) in the micromolar concentration range (2.4–8 µM). Four to eight biological replicates were performed.

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