Figure 4
From: Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
DNA double-strand breaks. The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay confirmed DNA double-strand breaks. The DNA double-strand breaks are indicated in green, and 4′,6-diamidino-2-phenylindole (DAPI) staining (blue) shows the nuclei. Control [(A) TUNEL assay; (B) DAPI staining; (C) merged, ×100]; slow-freezing [(D), TUNEL assay; (E) DAPI staining; (F) merged, ×200].
