Figure 1

Relative quantitative analysis of Hox and ParaHox gene transcripts during development of Dreissena rostriformis. (A) Localization of Hox genes on scaffolds is shown in the upper part. Identification numbers of scaffolds where genes were found and location of non-Hox genes identified within the cluster are indicated by red arrowheads. Hashtags mark genes for which no expression data by in situ hybridization could be produced. (B) Heat map shows relative normalized expression levels for each gene (Z-score). Genes expressed above the normalization threshold are depicted in graded shades of red, those below the threshold in shades of blue. Time after fertilization and corresponding developmental stages at 23 °C are to the left. Germ layers and major derivatives in animal schemes are depicted in grey (mesoderm), red (endoderm), and white (ectoderm), respectively. Asterisks mark the blastopore/mouth, sf indicates the shell field.