Figure 4
From: Identification of triptonide as a therapeutic agent for triple negative breast cancer treatment
Knockdown of Snail sensitizes TN-resistant HCC1806 cells to triptonide. (a) RT-qPCR (left) and western blot (right) analysis of SNAI knockdown in TN-resistant HCC1806 cells transfected with control siRNA, SNAI1 siRNA-1, or SNAI1 siRNA-2 for 48 h. Each bar is presented as mean ± SD (n = 3) (***, p < 0.001). (b) Cell growth assays using TN-resistant HCC1806 cells pre-transfected with control or SNAI1 siRNAs. Cells were grown in the presence of triptonide. Each bar is presented as mean ± SD (n = 3) (p > 0.05, **, p < 0.01). Western blot analysis of cleaved PARP levels in TN-resistant HCC1806 cells transfected with control or SNAI1 siRNAs. (c) Mammosphere formation assays in control and SNAI1-knockdown TN-resistant HCC1806 cells. Each bar is presented as mean ± SD (n = 3) (**, p < 0.01). Magnification, × 200.
