Figure 2
From: Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages
Delivery of genome-wide knockout library to iPSC-derived macrophages. (a) Western blot analysis of Cas9 expression in TKOv3-transduced cells using anti-Cas9 antibody detected an ~ 160 kDa band. GAPDH loading control. (b) Scatter plots comparing the abundance of each of the guides sequenced for each of the replicates. Pearson's correlation coefficient is reported for each pairwise comparison, with the correlation coefficient > 0.9 for each comparison. (c) Histogram showing the distribution of reads for the guides, with a mean of 339 reads per guide and a maximum of 2233 reads per guide. (d) Graph showing mapped sequences of the gDNA to the TKOv3 library. We found 89.1% of the sequences mapped to one guide in the library, 10.8% of the sequences were mapped to nothing in the library and 0.09% of the guides mapped to two or more guides in the library.
