Table 2 Antibodies (for flow cytometry and immunoblotting assays).
From: Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages
Antibody | Clone | Supplier | Cat. # | Dilution |
|---|---|---|---|---|
Flow cytometry | ||||
CD14-PE | HCD14 | Biolegend | 325605 | 1:20 |
IgG1Îş-PE isotype control | MOPC21 | Biolegend | 400113 | 1:20 |
CD11b-Alexa Fluor 647 | ICRF44 | Biolegend | 301319 | 1:20 |
CD80-Alexa Fluor 647 | 2D10 | Biolegend | 305215 | 1:20 |
IgG1Îş-Alexa Fluor 647 Isotype control | MOPC21 | Biolegend | 400130 | 1:20 |
CD163-FITC | GHI/61 | Biolegend | 333617 | 1:20 |
IgG1Îş-FITC isotype control | MOPC21 | Biolegend | 400109 | 1:20 |
TNF-Alexa Fluor 488 | MAb11 | BD Biosciences | 557722 | 1:5 |
IgG1Îş-Alexa Fluor 488 Isotype control | MOPC21 | BD Biosciences | 557721 | 1:5 |
Immunoblotting | ||||
Cas9 | 7A9-3A3 | Cell signaling | 14697 | 1:1000 |
SAMHD1 | Polyclonal | Abcam | ab67820 | 1:500 |
HPRT1 | Polyclonal | Proteintech | 15059-1-AP | 1:500 |
PPIB | EPR12703 | Abcam | ab178397 | 1:500 |
PPIB | Polyclonal | Abcam | ab16045 | 1:10 |
CDK4 | Polyclonal | BioLegend | 633301 | 1:500 |
CDK4 | Polyclonal | GeneTex | GTX102993 | 1:10 |
GAPDH | G-9 | scbt | sc-365062 | 1:1000 |
GAPDH | 14C10 | Cell signaling | 2118 | 1:500 |
Alexa Fluor 750 goat anti-mouse | Polyclonal | Thermo Fisher | A-21037 | 1:5000 |
Alexa Fluor 680 goat anti-rabbit | Polyclonal | Thermo Fisher | A27042 | 1:5000 |
Anti-Rabbit detection module for wes | Polyclonal | Bio-techne | DM-001 | ready-to-use |
