Table 1 Results of the PEX reaction with modified nucleotide substrates.
From: Mononucleotide repeat expansions with non-natural polymerase substrates
dU analog | dU1 | dU2 | dU3 | dU4 | dU5 | dU6 | dU7 | dU8 | dU9 | dU10 | dU11 |
|---|---|---|---|---|---|---|---|---|---|---|---|
M1 + dU variant | |||||||||||
Polymerase | Taq | ||||||||||
Nmaxa | 22 | 20 | 14 | 5 | 6 | 13 | 13 | 11 | 11 | 11 | 12 |
t, minb | 30 | 240 | 240 | 240 | 30 | 240 | 30 | 30 | 240 | 30 | 30 |
Polymerase | Vent (exo-) | ||||||||||
Nmax | 34 | 28 | 25 | 29 | 26 | 21 | 19 | 16 | 16 | 15 | 13 |
t, min | 5 | 5 | 30 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
Polymerase | Deep Vent (exo-) | ||||||||||
Nmax | 36 | 32 | 32 | 31 | 27 | 23 | 20 | 16 | 20 | 17 | 14 |
t, min | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
dU analog | dU1 | dU2 | dU3 | dU4 | dU5 | dU6 | dU7 | dU8 | dU9 | dU10 | dU11 |
|---|---|---|---|---|---|---|---|---|---|---|---|
M1 + dU variant + d(AGC) | |||||||||||
Polymerase | Taq | ||||||||||
Nmax | 14 | 6 | 6 | 5 | 5 | 5 | 8 | 8 | 7 | 11 | 10 |
t, min | 240 | 240 | 240 | 120 | 30 | 30 | 30 | 30 | 30 | 30 | 30 |
Polymerase | Vent (exo-) | ||||||||||
Nmax | 24 | 23 | 21 | 23 | 22 | 21 | 17 | 15 | 15 | 15 | 12 |
t, min | 5 | 5 | 30 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
Polymerase | Deep Vent (exo-) | ||||||||||
Nmax | 32 | 28 | 25 | 26 | 25 | 23 | 19 | 15 | 20 | 16 | 13 |
t, min | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
