Figure 3
From: Elucidation of the mechanism of subunit exchange in αB crystallin oligomers
AUC spectra of dilute and dense samples. (a) c(s20,w) from αB-crystallin in solution at 0.45 mg/mL measured at 60,000 rpm at 37 °C. The Mws of three peaks correspond to 19 kDa, 56 kDa and 101 kDa from the low c(s20,w) to the high one, respectively. The inset shows c(s20,w) from αB-crystallin in the solution at 0.45 mg/mL measured at 40,000 rpm at 37 °C. The pink and light blue arrows indicate the region corresponding to small molecular weight components and oligomers, respectively. (b) c(s20,w) from αB-crystallin in solution at 28.5 mg/mL measured at 60,000 rpm at 37 °C. Mws of three peaks correspond to 21 kDa, 53 kDa, and 87 kDa from the low c(s20,w) to the high one, respectively. The inset shows c(s20,w) from αB-crystallin in the solution at 28.5 mg/mL measured at 40,000 rpm at 37 °C. The pink and light blue arrows indicate the region corresponding to small molecular weight components and oligomers, respectively. This figure is prepared by the usage of IGOR Pro 6.34A (https://www.wavemetrics.com/forum/news-and-announcements/igor-634a-now-shipping).
