Figure 6

Flow cytometric analyses of dendritic cells (CD11c⁺, A), B cells (CD19⁺, B), cytotoxic T cells (CD8⁺, C), helper T cells (CD4⁺, D), and regulatory T cells (CD4⁺ CD25⁺ Foxp3⁺, E) in the spleens of DBA/1 mice with CIA. Three different spleens were randomly sampled in each group to analyze the T cell subsets. One-way ANOVA followed by Newman–Keuls post hoc test was used for comparisons of flow cytometric data among the groups. For comparisons between Anti-CD25 Ab and NOR Ab groups, and P60 and SCRAMB groups, paired Student’s t-test were performed. In all analyses, statistical differences were considered significant at p < 0.05. ^###p < 0.001 versus the NOR group, ^$$p < 0.01, ^$$$p < 0.001 versus the CIA group, and *p < 0.05, **p < 0.01, ***p < 0.001 versus the CIA_PLA2 group. ^$$p < 0.01 (paired Student´s t-test within between Anti-CD25 Ab and NOR Ab groups), and ^&&&P < 0.001 (paired Student´s t-test within between P60 and SCRAMB groups).