Figure 3 | Scientific Reports

Figure 3

From: Events associated with susceptibility to invasive Salmonella enterica serovar Typhi in BALB/c mice previously infected with Plasmodium berghei ANKA

Figure 3

Evaluation of phagocytic activity of ex vivo medullar neutrophils and macrophages. After 24 h of ex vivo incubation on glass slides of cells recovered from the femur of anesthetized mice from coinfected, monoinfected groups with P. berghei ANKA or S_Typhi and control group. After washing, the adherent cells were incubated with Phorbol 12-myristate 13-acetate (PMA) for activation in the phagocytosis assay. A suspension of S_Typhi killed by formalization was incubated with the adherent cells. Finally, the slides were washed, fixed in picric acid and stained using the Romanowsky method. Neutrophils and monocytes were visualized through staining characteristics and the total number of phagocytic bacteria was calculated. The phagocytic activity in cells between groups was compared using the percentage of phagocytes between adherent cells; % of neutrophils and % of macrophage containing phagocytic bacteria, respectively. The phagocytic activity in cells was compared by multiple comparison by Tukey’s method. The data are shown in a scattered plot with mean and standard deviations. Asterisks: level of significance of *p < 0.05, **p < 0.005 and ***p < 0.0005.

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