Table 2 Amount of PA5 in each PZP-purified fraction.

From: Purification of anti-glycoconjugate monoclonal antibodies using newly developed porous zirconia particles

Fraction

IgM (μg)

Recovery rate (%)

Total protein (mg)

IgM/total protein (%)

(a) 100 mM elution

Culture sup

51.4

n/a

17.03

0.3

Non-adsorbed

0.7

1.7

n.d

n.d

Wash

0.5

1.0

n.d

n.d

Elution

26.8

52.1

0.07

38.3

(b) 200 mM elution

Culture sup

52.7

n/a

17.03

0.3

Non-adsorbed

1.2

2.3

n.d

n.d

Wash

0.7

1.3

n.d

n.d

Elution

31.2

59.2

0.15

20.8

  1. PA5 monoclonal antibodies in hybridoma culture supernatants were purified with PZPs using 100 mM (a) or 200 mM (b) phosphate buffer (pH 8.0) as the elution buffer.
  2. sup supernatant, n/a not applicable, n.d. not determined.
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