Figure 1

Analytical sensitivity of the RABV-RPA assay. A semi-log regression analysis (A) was performed by plotting the RABV-RPA threshold time values against the number of RNA molecules detected in eight replicates (8/8). The RABV-RPA assay produced positive results between 3 and 10 min. Dilutions 10⁷ to 10 RNA molecules were detected 8 out of 8 runs and 1 molecule was not identified by the RT-RPA assay. The dots represent the mean values and the error bars represent the standard deviation. The probit regression analysis (B) was performed using data of eight RABV-RPA assay runs. The limit of detection at 95% probability (4 RNA molecules/reaction) is depicted by the red triangle. The graphs were plotted using PRISM (Graphpad Software Inc., San Diego, California).