Figure 2
From: The role of oxidised self-lipids and alveolar macrophage CD1b expression in COPD
CD1b expression in alveolar macrophages and 8-isporostane in the airways of COPD patients and smokers. (A) CD1b expression was assessed by flow cytometry using a FITC-conjugated antibody in alveolar macrophages from never-smoker controls (n = 14), ex-smokers (n = 12), current smokers (n = 7), and current- and ex-smoker COPD subjects (n = 29 and n = 36 respectively). Data presented as fold change relative to mean of never-smoker controls. p values vs control provided from Mann Whitney U test. (B) Representative confocal images showing increased CD1b (red) in alveolar macrophages from a COPD subject vs. never-smoker control. DAPI (blue); scale bar (μm). 8-isoprostane in (C) BALF from never-smoker controls (n = 16), current smokers (n = 11), and current- and ex-smoker COPD subjects (n = 16 and n = 15, respectively) and (D) EBC from never-smoker controls (n = 13), current smokers (n = 10), and current- and ex-smoker COPD subjects (n = 24 and n = 23, respectively). Data represents individual values and the mean. Significance from control at p < 0.05. Mann Whitney U test.
