Table 1 Gradient conditions for LC–MS analysis.

From: The role of oxidised self-lipids and alveolar macrophage CD1b expression in COPD

Time (min)

Flow rate (mL/min)

% A

Curve

0

0.4

60

–

2

0.4

57

6

2.1

0.4

50

1

12

0.4

46

6

12.1

0.4

30

1

18

0.4

1

6

20.4

0.4

1

6

20.5

0.4

60

1

22.5

0.4

60

1

  1. Online MS analysis was performed using a Xevo G2-XS Q-ToF (Waters Corporation, Manchester) in positive ion sensitivity mode over the m/z range 120–1200.
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