Figure 4
From: DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice
Antibody quantification and neutralization of the vaccinated mice. (A) Mice were immunized with 10 μg of candidate vaccines as indicated on the graph and serial dilutions of serum from vaccinated mice were assessed for neutralization of SARS-CoV-2 pseudotyped lentiviruses harboring a luciferase reporter gene. The ID50 titers were calculated as the reciprocal dilution where infection (RLU) was reduced by 50% relative to infection in the absence of serum. The geometric mean ID50 for each group is displayed. (B) Neutralization curves depicting percent neutralization against serum dilution. (C) Curves depicting OD490 readouts in anti-RBD ELISA assay detecting IgG binding antibodies in the serum of immunized mice. Across all groups, RBD-specific IgG titers correlated strongly with pseudovirus neutralization. Statistical analysis was performed comparing all groups except 1xDREP-Secto using Kruskal Wallis test followed by Dunn’s test for multiple comparisons, *p < 0.05.
