Figure 5
From: DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice
Analysis of S1-specific T cell responses. (A) Schematic representation of the experiment schedule. Mice were immunized on days 1 (prime) and 28 (boost) as indicated in the graph and spleens were collected 10 days after each immunization. (B) Quantification of IFN-γ secreting splenocytes upon restimulation with SARS-CoV-2 peptides. T cell responses were analyzed by ELISpot after restimulation of splenocytes with the SARS-CoV-2 S1 peptides (15-mers overlapping with 11 amino acids), covering the S1 domain of the spike glycoprotein. Pool 1, SARS-CoV-2 S1 peptides 1–83; pool 2, SARS-CoV-2 S1 peptides 84–166. Statistical analysis was performed comparing all groups except naïve controls using Kruskal Wallis test followed by Dunn’s test for multiple comparisons, *p < 0.05; **p < 0.01.
