Table 1 Relative distribution of aminopeptidase activity in the P. falciparum cellular fractions (C1, C2, V1 and V2) calculated from a standard curve prepared using free NMMec.

From: Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (PfM1AAP) and M17 leucyl aminopeptidase (PfM17LAP)

Fraction

H-Arg-NHMec

H-Leu-NHMec

Enzyme activity (Unitsa)

Relative activity (%)

Enzyme activity (Unitsa)

Relative activity (%)

Total

178 ± 15.6

100

133.6 ± 1.1

100

Cytosolic 1 (C1)

145.5 ± 19.6

80 ± 7.5

116.4 ± 17.3

85 ± 10.5

Cytosolic 2 (C2)

13 ± 9.3

6.8 ± 4.3

8.4 ± 7.9

5.8 ± 4.9

Vacuolar 1 (V1)

17.5 ± 12.6

9.4 ± 6.7

11.3 ± 8.7

8.3 ± 6.9

Vacuolar 2 (V2)

6.4 ± 5.2

3.8 ± 3.3

1.3 ± 1.5

1.1 ± 1.2

  1. aOne unit of enzyme is defined as the nanomoles of NHMec released per 30 min at 37 °C.
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