Figure 4
From: The G127V variant of the prion protein interferes with dimer formation in vitro but not in cellulo
Diminished heterodimer formation in case of a G126V mutation in mPrP. The amount of crosslinked heterodimers are assessed for the indicated mCherry-tagged and untagged prion protein mixtures, using two different positions for the pBpa (denoted as “(X)”) substitution (Y127pBpa and S131pBpa) in untagged mPrP. The values represent percentages calculated based on gel densitometry analysis as described in Materials and Methods. White bars indicate values obtained in case of control samples containing no pBpa. Black and grey color bars indicate the % of heterodimers without and with Val mutation at position 126, respectively, in either mPrP or mPrP-mCh proteins. Statistical significances of the differences were calculated by unpaired t-test. Significantly different pairs are marked on the graph, and significance levels are indicated by stars, *p ≤ 0.05. “(X)” stands for pBpa in the notation of Y-axis category names referring to the pBpa-mutation at the indicated positions.
