Figure 2
The correlation between structural disorder and organism complexity. (A) Protein disorder degree was measured by the average of all representative proteins (the longest one for each gene) in each species. (B) Proteins with more than 30% disordered amino acids (PSDR > 30%) were regarded as disordered proteins. Protein disorder degree was measured by the percentage of disordered proteins in each species. (C, D) At domain level, structural disorder of each species was measured by the percentage of IDDs (C, DSDR > 30%, or D, CDRN ≥ 1). (E, F) At domain family level, domain families were defined using dominant category method; structural disorder of each species was measured by the percentage of intrinsically disordered domain families (E, family_DSDR > 30%, or F, family_CDRN ≥ 1). Scatter plots was made for organism complexity and structural disorder values obtained by different methods in fifty-one eukaryotes. Spearman method was used for the correlation analysis. The correlation coefficients (R) and P values (P) are shown in the inset, among which the significant results are shown as red. (G) Box-plot of the percentage of intrinsically disordered (DSDR > 30%) domains (left panel) or domain families (right panel) in each species across the different phyla of eukaryotes. The four kingdoms of eukaryotes are marked on the bottom of the figure. The values of upper and lower quartile, and the median values are indicated as bars in the boxes. The differences in percentage of disordered domain distribution between adjacent categories are examined by Mann–Whitney U test. The corrected P values are marked as stars in sub-figure G (*P < 0.05; ** P < 0.01).
